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KMID : 0380420180420020061
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Journal of Prventive Veterinary Medicine
2018 Volume.42 No. 2 p.61 ~ p.65
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Anti-inflammatory activity of doxorubicin on lipopolysaccharide-induced hyperactivation of spleen cells
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Jang Ye-Ryoung
Yoon Hye-Rin
Kim Mi-Hyoung
Joo Hong-Gu
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Abstract
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Doxorubicin has been used to treating cancers, including breast cancer, bladder cancer, and acute lymphocytic leukemia, however, few studies have investigated its anti-inflammatory activity. In this study, we used mouse spleen cells treated with lipopolysaccharide (LPS), a representative inflammatory agent to investigate the effects of doxorubicin. Specially, we investigated the effects of doxorubicin on metabolic activity, cell size, cell death, and cytokine production of LPS-treated spleen cells. Doxorubicin significantly decreased the metabolic activity, even when applied at relatively low concentrations (1.6-8 ng/mL). To investigate the potential mechanism, we measured the mitochondrial membrane potential (MMP) of the LPS-treated spleen cells using Rhodamine 123. Doxorubicin decreased MMP and cell size, and induced cell death. Furthermore, doxorubicin suppressed the production of tumor necrosis factor (TNF)-alpha, a representative cytokine, in LPS-treated spleen cells. Taken together, doxorubicin decreased metabolic activity and the production of inflammatory cytokines, while increasing the death of LPS-induced hyperactivated spleen cells. This results will enable broader application of doxorubicin, as an anti-inflammatory agent, in clinical and research fields.
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KEYWORD
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Doxorubicin, spleen cells, lipopolysaccharide, TNF-alpha
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